Normalization Constant Calculation for Quantitative EPR Analysis
Source:R/quantify_EPR_Norm_const.R
quantify_EPR_Norm_const.RdNormalization constant used by quantify_EPR_Abs or to normalize
the EPR spectrum intensity. Calculation depends on the receiver gain expression.
Usage
quantify_EPR_Norm_const(
conv.time.ms,
Nscans,
Npoints = NULL,
Bsw = NULL,
rg,
rg.unit = "dB"
)Arguments
- conv.time.ms
Numeric, conversion time in milliseconds.
- Nscans
Numeric, number of scans.
- Npoints
Numeric, number of points (resolution) corresponding to individual sweep, if
rg.unit = "unitless"(rg.unit = "Unitless"). Default:Npoints = NULL.- Bsw
Numeric, experimental sweep width (magnetic flux density recording region, \(B_{\text{SW}}\)) in "G" if
rg.unit = "unitless"(rg.unit = "Unitless"). Default:Bsw = NULL.- rg
Numeric, receiver gain value.
- rg.unit
Character string corresponding to unit of the receiver gain. Either
rg.unit = "db"(rg.unit = "dB", default) orrg.unit = "unitless"(rg.unit = "Unitless").
Details
For the receiver gain expressed in \(\text{dB}\) units the normalization constant is defined
by the following relation =>
$$N_{\text{norm}} = t_{\text{C}}(\text{ms})\,N_{\text{Scans}}\,(20)\,
10^{(G_{\text{R}}(\text{dB})/20)}$$
where \(t_{\text{C}}(\text{ms})\) depicts the conversion time in \(\text{ms}\);
\(N_{\text{Scans}}\) corresponds to number of scans and \(G_{\text{R}}(\text{dB})\)
is the receiver gain in \(\text{dB}\). In the case that the receiver gain is unitless, the normalization
constant is defined by =>
$$N_{\text{norm}} = t_{\text{C}}(\text{ms})\,G_{\text{R}}\,
(N_{\text{points}} - 1)\,N_{\text{scans}}\,/\,B_{\text{SW}}(\text{G})$$
where \(G_{\text{R}}\) and \(B_{\text{SW}}(\text{G})\) correspond to unitless receiver gain
and sweep width in Gauss, respectively. \(N_{\text{points}}\) equals to the number of points (resolution of
an individual sweep). One can gather all parameters
by the readEPR_param_slct or by the readEPR_params_tabs function from
the corresponding .DSC/.dsc or .par file. If during recording of EPR spectra
the option Normalize Acquisition (in Spectrometer Configuration/Acquisition Options)
is activated, THE INTENSITY is ALREADY NORMALIZED and DOESN'T REQUIRE ANY ADDITIONAL
NORMALIZATION !. Please, refer also to the quantify_EPR_Abs function.
References
Weber RT (2011). Xenon User's Guide. Bruker BioSpin Manual Version 1.3, Software Version 1.1b50.
Bruker Biospin (2007). WIN-EPR User's Manual.
See also
Other Evaluations and Quantification:
eval_integ_EPR_Spec(),
eval_kinR_EPR_modelFit(),
eval_kinR_ODE_model(),
quantify_EPR_Abs()